Dell 3100 Especificações descarregar pdf (Página 183)

Fluid Imaging

Operating Principles

Rev. D Dimension 3100 Manual 163

Note: The difference between the vertical deﬂection before engaging and the setpoint

is related to the amount of force that the cantilever probe tip applies to the

sample.

Note: Verify that there is not too large a difference between the setpoint and the

vertical deflection signal before engaging. Samples are typically softer in liquid

than in air. It may be desirable to reduce the setpoint once engaged to obtain the

minimum tracking force.

Adjust Photodetector

1. Adjust the photodetector so that the red dot moves toward the center of the laser alignment

window using the two photodetector adjustment knobs located on the side of the Dimension

head.

2. Set the Vertical deflection to 0.

3. Verify that the red dot is centered and elliptical in shape in the laser alignment window.

10.5.12 Locate Tip

1. Using the mouse, select Locate Tip from under the Stage pop-down menu or click the

Locate Tip icon.

2. Center the tip on the cantilever under the cross hairs using the two adjustment screws located

to the left of the optical objective on the microscope.

3. Focus on the tip end of the cantilever using the trackball while holding the bottom left button.

Note: See Chapter 7 for more detailed instructions for locating the tip.

10.5.13 Focus Surface

When focusing on the sample surface in air before adding ﬂuid to the sample area, the following

procedure is necessary:

1. Align laser

2. Locate tip

3. Focus surface

4. Add fluid

5. Readjust laser alignment

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