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Fluid Imaging

Operating Principles

160 Dimension 3100 Manual Rev. D

10.5.7 “False” Reﬂections

The cantilever substrate rests on a smooth, angled, glass surface. The laser reﬂects off the angled

glass surface resulting in a visible laser reﬂection on the laser viewing window on the microscope

head, even when the laser is not aimed at the cantilever (see Figure 10.5b). This reﬂection from the

glass surface does not affect the operation of the AFM, but it can be a source of confusion when

aligning the laser on the cantilever. Ignore this fainter reﬂection and look instead for the much

brighter reﬂection off the cantilever.

Figure 10.5b False Reﬂections from Fluid Tip Holder

Once the ﬂuid tip holder is plugged into the bottom of the AFM scanner, note the faint laser

reﬂection visible on the glass in the tip holder (in the laser viewing window).

Note: The SUM signal on the display monitor will typically show less than 1V when

the laser is not yet aligned on the cantilever. The SUM signal should rise above

1V when the laser is truly reﬂecting off the cantilever.

10.5.8 Load Sample

Prepare the Sample for Imaging

Set the sample on the microscope stage. For TappingMode in ﬂuid, use a petri dish, or if the sample

is hydrophobic, image the surface within a drop of ﬂuid.

Note: Immersed samples may tend to dry out during imaging due to evaporation;

therefore, keep a quantity of ﬂuid nearby to periodically replenish lost ﬂuid.

false reflection

laser reflects off glass

laser beam

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