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Fluid Imaging

Operating Principles

Rev. D Dimension 3100 Manual 161

Remount the Dimension Head

With the sample prepared and positioned on the stage, slowly remount the Dimension head in its

dovetailed, vertical mount.

Ideally, the Dimension head should come to rest in its mount at a level where the tip is just above

the level of the ﬂuid. If the head rests too low in its mount, use either of the following methods to

raise the mount:

• Raise the mount further by using the Motor > Step Motor > Tip Up command.

• Remove the head from its mount and lower the sample into the ﬂuid level. Remount the

head.

Note: Because the tip is lowered through a ﬂuid layer, the laser beam will refract

slightly requiring minor adjustments. Refer to Section 10.5.10 for more

information regarding aiming the laser through a ﬂuid layer.

At this point, the ﬂuid cell should be prepared for TappingMode imaging: the cantilever is aligned

with the laser, the sample is prepared and the tip is immersed in ﬂuid and positioned one millimeter

above the sample surface. All that remains is to tune the cantilever.

Verify that the Microscope is Dry

Verify that all Dimension 3100 surfaces are free of spilled ﬂuid. Wick away moisture and droplets

with ﬁlter paper.

CAUTION: If the fluid level is too high, the end of the scanner tube may be

dunked too far into fluid. Do not allow this to happen. Permanent

damage to the scanner tube may occur.

ATTENTION: Si le niveau du liquide est trop important, l’extrémité des

céramiques piézo-électriques peut tremper dans le liquide. Ceci ne

doit jamais arriver. Ce cas de ﬁgure peut entraîner des dommages

déﬁnitifs sur les céramiques piézo-électriques.

VORSICHT: Falls der Flüssigkeitsspiegel zu hoch ist, könnte das Ende des

Scannerröhrchens in die Flüssigkeit eintauchen-dies darf auf

keinen Fall passieren. Es könnte sonst eine dauerhafte Schädigung

des Scannerröhrchens eintreten.

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